Broad distribution of enterotoxin genes (hblCDA, nheABC, cytK, and entFM) among Bacillus thuringiensis and Bacillus cereus as shown by novel primers.

@article{Ngamwongsatit2008BroadDO,
  title={Broad distribution of enterotoxin genes (hblCDA, nheABC, cytK, and entFM) among Bacillus thuringiensis and Bacillus cereus as shown by novel primers.},
  author={Puriya Ngamwongsatit and Wasin Buasri and Panuwat Pianariyanon and Chaiwat Pulsrikarn and Michio Ohba and Apinya Assavanig and Watanalai Panbangred},
  journal={International journal of food microbiology},
  year={2008},
  volume={121 3},
  pages={352-6}
}
Eight new pairs of PCR primers were designed and efficiently detect eight toxin genes (hblC, hblD, hblA, nheA, nheB, nheC, cytK, and entFM) in 411 B. cereus strains (121 food- and 290 soil isolates) and 205 B. thuringiensis strains (43 serovars, 10 food- and 152 soil isolates). According to the presence of these eight toxin genes, they were divided into four groups among the total 616 isolates. In Group I, all eight genes occurred simultaneously in 403 (65.42%) isolates, while Group II (134… CONTINUE READING

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