Bright sub-20-nm cathodoluminescent nanoprobes for electron microscopy

  title={Bright sub-20-nm cathodoluminescent nanoprobes for electron microscopy},
  author={Maxim B. Prigozhin and Peter C. Maurer and Alexandra M Courtis and Nian Liu and Michael D. Wisser and Chris Siefe and Bining Tian and Emory M. Chan and Guosheng Song and Stefan Fischer and Shaul Aloni and D. Frank Ogletree and Edward S. Barnard and Lydia-Marie Joubert and Jianghong Rao and A. Paul Alivisatos and Roger M. Macfarlane and Bruce E. Cohen and Yi Cui and Jennifer A. Dionne and Steven Chu},
  journal={Nature Nanotechnology},
Electron microscopy has been instrumental in our understanding of complex biological systems. Although electron microscopy reveals cellular morphology with nanoscale resolution, it does not provide information on the location of different types of proteins. An electron-microscopy-based bioimaging technology capable of localizing individual proteins and resolving protein–protein interactions with respect to cellular ultrastructure would provide important insights into the molecular biology of a… Expand
19 Citations
Correlative Cathodoluminescence Electron Microscopy: Immunolabeling Using Rare-Earth Element Doped Nanoparticles.
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Ultra-Bright and Stable Luminescent Labels for Correlative Cathodoluminescence Electron Microscopy (CCLEM) Bioimaging.
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Lanthanide-Doped Upconversion Nanoparticles for Super-Resolution Microscopy
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ColorEM: analytical electron microscopy for element-guided identification and imaging of the building blocks of life
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Correlative cathodoluminescence electron microscopy bioimaging: towards single protein labelling with ultrastructural context.
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Electron-Beam Induced Luminescence and Bleaching in Polymer Resins and Embedded Biomaterial.
These findings provide perspective to direct electron-beam excitation approaches like cathodoluminescence that may be obscured by these nonspecific electron-induced signals, and are advantageous for turning many polymers into luminescent nanostructure or to fluorescently visualize (micro)plastics. Expand
Lanthanide-Based Nanosensors: Refining Nanoparticle Responsiveness for Single Particle Imaging of Stimuli.
A perspective provides a roadmap for translating ensemble LNP sensing capabilities to the single particle level, enabling nanometer-scale sensing in biology, medicine, and sustainability. Expand
Detection and Localization of Eu on Biosilica by Analytical Scanning Electron Microscopy
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Dynamic upconversion multicolour editing enabled by molecule-assisted opto-electrochemical modulation
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Single-particle spectroscopy for functional nanomaterials
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Multicolor Electron Microscopy for Simultaneous Visualization of Multiple Molecular Species.
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Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots
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Multicolor Cathodoluminescence Microscopy for Biological Imaging with Nanophosphors
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Upconversion luminescence imaging of cells and small animals
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Optical excitations in electron microscopy
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