Bright monomeric photoactivatable red fluorescent protein for two-color super-resolution sptPALM of live cells.

@article{Subach2010BrightMP,
  title={Bright monomeric photoactivatable red fluorescent protein for two-color super-resolution sptPALM of live cells.},
  author={Fedor V. Subach and George H. Patterson and Malte Renz and Jennifer Lippincott-Schwartz and Vladislav V Verkhusha},
  journal={Journal of the American Chemical Society},
  year={2010},
  volume={132 18},
  pages={6481-91}
}
Rapidly emerging techniques of super-resolution single-molecule microscopy of living cells rely on the continued development of genetically encoded photoactivatable fluorescent proteins. On the basis of monomeric TagRFP, we have developed a photoactivatable TagRFP protein that is initially dark but becomes red fluorescent after violet light irradiation. Compared to other monomeric dark-to-red photoactivatable proteins including PAmCherry, PATagRFP has substantially higher molecular brightness… CONTINUE READING
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