Brief exposure to cycloheximide prior to electrical activation improves in vitro blastocyst development of porcine parthenogenetic and reconstructed embryos.

@article{Naruse2007BriefET,
  title={Brief exposure to cycloheximide prior to electrical activation improves in vitro blastocyst development of porcine parthenogenetic and reconstructed embryos.},
  author={K. Naruse and Y. Quan and B. C. Kim and Ji Hyun Lee and C. S. Park and D. I. Jin},
  journal={Theriogenology},
  year={2007},
  volume={68 5},
  pages={
          709-16
        }
}
To investigate the effects of cycloheximide exposure before electrical activation of in vitro-matured porcine oocytes on the subsequent development of parthenogenetic embryos, cumulus-free mature oocytes were exposed to NCSU-23 medium containing cycloheximide (10 microg/mL) for 0, 5, 10, 20, 30 and 60 min, activated by electrical pulse treatment (1.5 kV/cm, 100 micros) and then cultured in PZM-3 for 7 days. To evaluate the effects of cycloheximide on the activation of nuclear transfer embryos… Expand
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References

SHOWING 1-10 OF 36 REFERENCES
Parthenogenetic development of bovine oocytes matured in vitro for 24 hr and activated by ethanol and cycloheximide
TLDR
The combined ethanol and cycloheximide treatment supported high rates of parthenogenetic development using 24 hr IVM bovine oocytes and blastocyst rate was significantly higher when cytochalasin B was added to the combined activation regimen. Expand
Inactivation of MPF and MAP kinase by single electrical stimulus for parthenogenetic development of porcine oocytes
TLDR
The results indicate that the optimal single electrical pulse is effective on the inactivation of MPF and MAP kinase, eventually leading to the parthenogenetic development of porcine oocytes. Expand
Parthenogenetic development of porcine oocytes treated by ethanol, cycloheximide, cytochalasin B and 6-dimethylaminopurine.
TLDR
The optimal activation treatment of ethanol exposure alone for the in vitro matured porcine oocytes was 8% ethanol for 8-15 min, and the rate of blastocyst formation was higher in the ethanol+CHX+CCB+6-DMAP treatment than in other treatments. Expand
Optimization of parthenogenetic activation protocol in porcine
TLDR
The results demonstrated that a single 30 μsec pulse of 2.2 kV/cm DC followed by culturing in post‐activation medium with CB for 5 hr were effective parameters for parthenogenetic activation and blastocyst formation of in vitro matured porcine oocytes which suggests that asingle calcium rise is sufficient to activate pig oocytes and to achieve high rate of blastocySt development. Expand
Nuclear dynamics of parthenogenesis of bovine oocytes matured in vitro for 20 and 40 hours and activated with combined ethanol and cycloheximide treatment
TLDR
The results demonstrated that the combined activation treatment effectively drove the IVM oocytes, both young (20 hr) and aging (40 hr), out of metaphase arrest and nuclear events in aging oocytes proceeded faster than those in young ones. Expand
Development of cloned pig embryos by nuclear transfer following different activation treatments
TLDR
The results suggested that although DMAP enhanced development and higher cell number, due attention should be paid to abnormal ploidy in pig NT embryos. Expand
Activation of in vitro matured pig oocytes by combined treatment of ethanol and cycloheximide
TLDR
Investigation of the combined effects of exposure to ethanol and to the protein synthesis inhibitor cycloheximide, on activation of in vitro-matured pig oocytes found a maximal activation rate was observed when oocytes were treated with 10% ethanol for 1 min and subsequently cultured with cyclo heximide. Expand
Age dependence of bovine oocyte activation.
TLDR
Maturational age of the oocyte was the main determinant of activation ability, and maximum response to the two activating stimuli occurred in oocytes at similar maturational ages. Expand
Activation of pig oocytes using calcium ionophore: effect of protein synthesis inhibitor cycloheximide.
TLDR
In vitro matured pig oocytes were activated using a combined treatment of calcium ionophore A 23187 with cycloheximide and the percentage of oocytes that were able to develop after activation significantly increased and the highest activation rate was observed after treatment with 50 microM Ionophore. Expand
Improvement of an Electrical Activation Protocol for Porcine Oocytes1
TLDR
The formation of parthenogenetic blastocysts was optimal when activation was at 44 h of maturation using three 80-μsec consecutive pulses of 1.0 kV/cm DC, implying a potential for further in vivo development. Expand
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