Bridging the gaps in design methodologies by evolutionary optimization of the stability and proficiency of designed Kemp eliminase KE59.

@article{Khersonsky2012BridgingTG,
  title={Bridging the gaps in design methodologies by evolutionary optimization of the stability and proficiency of designed Kemp eliminase KE59.},
  author={Olga Khersonsky and Gert Kiss and Daniela R{\"o}thlisberger and Orly Dym and Shira Albeck and Kendall N Houk and David Baker and Dan S. Tawfik},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2012},
  volume={109 26},
  pages={10358-63}
}
Computational design is a test of our understanding of enzyme catalysis and a means of engineering novel, tailor-made enzymes. While the de novo computational design of catalytically efficient enzymes remains a challenge, designed enzymes may comprise unique starting points for further optimization by directed evolution. Directed evolution of two computationally designed Kemp eliminases, KE07 and KE70, led to low to moderately efficient enzymes (k(cat)/K(m) values of ≤ 5 10(4) M(-1)s(-1)). Here… CONTINUE READING

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