Bridging the Resolution Gap: Imaging the Same Transcription Factories in Cryosections by Light and Electron Microscopy

@article{Pombo1999BridgingTR,
  title={Bridging the Resolution Gap: Imaging the Same Transcription Factories in Cryosections by Light and Electron Microscopy},
  author={Ana Pombo and Michael S. Hollinshead and Peter R. Cook},
  journal={Journal of Histochemistry & Cytochemistry},
  year={1999},
  volume={47},
  pages={471 - 480}
}
  • Ana Pombo, Michael S. Hollinshead, Peter R. Cook
  • Published in
    The journal of histochemistry…
    1999
  • Biology, Medicine
  • Journal of Histochemistry & Cytochemistry
  • The resolution of conventional light microscopy is limited to ∼200 nm in the x- and y-axes and <500 nm in the z-axis. A simple way of improving z-axis resolution is to analyze thin sections of 100–200 nm. The utility of such an approach is illustrated by reference to transcription sites imaged in cryosections of human nuclei. Cells are permeabilized, allowed to extend nascent transcripts in Br-UTP, fixed, cryosectioned, and Br-RNA-immunolabeled with fluorochromes and gold particles. As expected… CONTINUE READING

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