Bricks and blueprints: methods and standards for DNA assembly

@article{Casini2015BricksAB,
  title={Bricks and blueprints: methods and standards for DNA assembly},
  author={Arturo Casini and M. Storch and Geoff S. Baldwin and Tom Ellis},
  journal={Nature Reviews Molecular Cell Biology},
  year={2015},
  volume={16},
  pages={568-576}
}
DNA assembly is a key part of constructing gene expression systems and even whole chromosomes. In the past decade, a plethora of powerful new DNA assembly methods — including Gibson Assembly, Golden Gate and ligase cycling reaction (LCR) — have been developed. In this Innovation article, we discuss these methods as well as standards such as the modular cloning (MoClo) system, GoldenBraid, modular overlap-directed assembly with linkers (MODAL) and PaperClip, which have been developed to… Expand

Paper Mentions

DNA Assembly for Plant Biology.
  • N. Patron
  • Biology, Medicine
  • Current protocols in plant biology
  • 2016
TLDR
The availability of collections of DNA parts and molecular toolkits that allow the facile assembly of multigene binary constructs and the establishment of standards for the creation of new parts means Type IIS-mediated assembly has become a powerful technology for modern plant molecular biologists. Expand
Universal loop assembly: open, efficient and cross-kingdom DNA fabrication
TLDR
‘universal Loop’ (uLoop) assembly is described, a system based on Loop assembly for use in potentially any organism of choice, that retains high efficiency regardless of the final host. Expand
BASIC: A Simple and Accurate Modular DNA Assembly Method.
TLDR
The modularity within the BASIC framework is further extended by the possibility of encoding ribosomal binding sites (RBS) and peptide linker sequences directly on the linkers used for assembly, making BASIC a highly versatile library construction method for combinatorial part assembly. Expand
Mobius Assembly: a versatile framework for Golden Gate assembly
TLDR
A new Golden Gate Assembly framework called Mobius Assembly is developed, which combines vector toolkit simplicity with high cloning capacity and embraces the standard overhangs defined by MoClo and Golden Braid to confer exchangeability, but with reduced domestication requirement. Expand
C-Brick: A New Standard for Assembly of Biological Parts Using Cpf1.
TLDR
A new DNA assembly standard, namely C-Brick, which employs the newly identified class 2 type V CRISPR-Cas systems protein Cpf1 endonuclease, and integrates both advantages of long recognition sites and short scars. Expand
Universal Loop assembly (uLoop): open, efficient, and species-agnostic DNA fabrication
TLDR
“universal Loop” (uLoop) assembly is described, a simple system based on Loop assembly that enables hierarchical fabrication of large DNA constructs (> 30 kb) for any organism of choice and decouples efficiency of assembly from the stability of vectors in the target organism. Expand
DNA-BOT: A low-cost, automated DNA assembly platform for synthetic biology
Multi-part DNA assembly is the physical starting point for many projects in Synthetic and Molecular Biology. The ability to explore a genetic design space by building extensive libraries of DNAExpand
BASIC: A Simple and Accurate Modular DNA Assembly Method.
TLDR
BASIC is a versatile assembly method for combinatorial and complex assemblies both at bench and biofoundry scale, and relies on a single tier format, which provides for easy hierarchical assembly, and is highly accurate for up to seven parts per assembly round. Expand
DNA-BOT: a low-cost, automated DNA assembly platform for synthetic biology
TLDR
An open-source software package is developed and the performance of DNA-BOT is demonstrated by simultaneously assembling 88 constructs composed of 10 genetic parts, evaluating the promoter, ribosome binding site and gene order design space for a three-gene operon. Expand
Rapid 40 kb genome construction from 52 parts
TLDR
This work carried out rapid assembly of the 40 kb T7 bacteriophage genome from 52 parts and recovering infectious phage particles after cellular transformation, using the new Golden Gate assembly protocols and design principles described here. Expand
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