Brefeldin A as a regulator of grp78 gene expression in mammalian cells.

@article{Liu1992BrefeldinAA,
  title={Brefeldin A as a regulator of grp78 gene expression in mammalian cells.},
  author={Eric Liu and Jing-Hsiung James Ou and Amy Si-Ying Lee},
  journal={The Journal of biological chemistry},
  year={1992},
  volume={267 10},
  pages={7128-33}
}
We report here that brefeldin A (BFA), which specifically blocks protein transport from an endoplasmic reticulum to the Golgi apparatus and causes resorption of Golgi membrane to the endoplasmic reticulum, specifically induced the endoplasmic reticulum-resident protein GRP78. Treatment of a human hepatoma cell line Alex-PC with BFA at a concentration of 5 micrograms/ml increased the grp78 transcript level by 12-fold. Analyses of the transcriptional rate of grp78 and the transfection with grp78… CONTINUE READING