Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

@article{Boch2009BreakingTC,
  title={Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors},
  author={Jens Boch and Heidi Scholze and Sebastian Schornack and Angelika Landgraf and Simone Hahn and Sabine Kay and Thomas Lahaye and Anja Nickstadt and Ulla Bonas},
  journal={Science},
  year={2009},
  volume={326},
  pages={1509 - 1512}
}
TAL Order Xanthomonas bacteria attack their plant hosts by delivering their own transcription-activator–like (TAL) proteins into the plant cell nucleus and alter the plant's gene regulation (see the Perspective by Voytas and Joung). Moscou and Bogdanove (p. 1501, published online 29 October: see the cover) and Boch et al. (p. 1509, published online 29 October) have now discovered how the similar but not identical repeats in the TAL proteins encode the specificity needed for the proteins to find… 

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A method called "Golden TAL Technology" is developed that allows a flexible assembly of TAL proteins with a designed order of repeats, which allows a simple reprogramming of DNA-binding specificity.

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An overview of the current tools and strategies that may be applied for finding targets of TAL effectors is given and several perspectives offered by these new tools are highlighted.

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This work reviewed the development of this technology in multi-level and multi species, and its advantages and disadvantages compared with ZFNs and CRISPR/Cas technology, and addressed its special advantages in industrial microbe breeding, vector construction, targeting precision, high efficiency of editing and biological safety.

The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target

The crystal structure of PthXo1 bound to its DNA target was determined by high-throughput computational structure prediction and validated by heavy-atom derivatization, and illustrates the basis of TAL effector–DNA recognition.

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[Molecular recognition code between pathogenic bacterial TAL-effectors and host target genes: a review].

The findings and functions of TAL effectors, the binding specificity and recognition code between TAL-effectors and host target genes, and the possible applications and future prospects of the molecular recognition code have been discussed.
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