Breaking symmetry in the structure determination of (large) symmetric protein dimers.

@article{Gaponenko2002BreakingSI,
  title={Breaking symmetry in the structure determination of (large) symmetric protein dimers.},
  author={Vadim Gaponenko and Amanda S. Altieri and Jess Li and Richard Byrd},
  journal={Journal of biomolecular NMR},
  year={2002},
  volume={24 2},
  pages={143-8}
}
We demonstrate a novel methodology to disrupt the symmetry in the NMR spectra of homodimers. A paramagnetic probe is introduced sub-stoichiometrically to create an asymmetric system with the paramagnetic probe residing on only one monomer within the dimer. This creates sufficient magnetic anisotropy for resolution of symmetry-related overlapped resonances… CONTINUE READING