BrdU immunohistochemistry for studying adult neurogenesis: Paradigms, pitfalls, limitations, and validation

@article{Taupin2007BrdUIF,
  title={BrdU immunohistochemistry for studying adult neurogenesis: Paradigms, pitfalls, limitations, and validation},
  author={Philippe Taupin},
  journal={Brain Research Reviews},
  year={2007},
  volume={53},
  pages={198-214}
}
  • P. Taupin
  • Published 31 January 2007
  • Biology
  • Brain Research Reviews
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Genetic Detection of Neurogenesis and Astrocytic Transformation of Radial Glia
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Flow cytometric analysis of BrdU incorporation as a high-throughput method for measuring adult neurogenesis in the mouse.
Synthetic Thymidine Analog Labeling without Misconceptions
TLDR
The results indicate the potential caveats in labeling the replicating DNA using thymidine analogs and suggest guidelines for applying this approach and show that, in a wide range of doses, EdU and BrdU label similar numbers of cells in the dentate gyrus shortly after administration.
Bromodeoxyuridine inhibits cancer cell proliferation in vitro and in vivo.
TLDR
The results suggest that BrdU may have an important role as an adjunctive therapeutic for a wide variety of cancers based on new insights into its effect as a negative regulator of cell cycle progression.
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TLDR
Evidence is presented that BrdU is not significantly incorporated during DNA repair and that labeling is not detected in vulnerable or dying postmitotic neurons, even when a high dose of Brd U is directly infused into the brain.
Bromodeoxyuridine administered during neurogenesis of the projection neurons causes cerebellar defects in rat
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Bromodeoxyuridine is a useful tool to study neural development, but its cytotoxicity represents a serious pitfall particularly when multiple doses are used to label cells.
Hypoxia-Ischemia Induces DNA Synthesis without Cell Proliferation in Dying Neurons in Adult Rodent Brain
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The combination of hypoxia and ischemia induces adult rodent neurons to resume DNA synthesis as indicated by incorporation of bromodeoxyuridine (BrdU) and expression of G1/S-phase cell cycle transition markers and the demonstration of neurogenesis after brain injury requires not only BrdU uptake and mature neuronal markers but also evidence showing absence of apoptotic markers.
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A high dose of BrdU (300 mg/kg) is shown to be a specific, quantitative, and nontoxic marker of dividing cells in the adult rat dentate gyrus, whereas lower doses label only a fraction of the S‐phase cells.
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