Branching Network of Proteinaceous Filaments within the Parasitophorous Vacuole of Encephalitozoon cuniculi and Encephalitozoon hellem

  title={Branching Network of Proteinaceous Filaments within the Parasitophorous Vacuole of Encephalitozoon cuniculi and Encephalitozoon hellem},
  author={Kaya Ghosh and Eddie N. Laboy Nieves and Patrick J. Keeling and Jean-François Pombert and Philipp P Henrich and Ann Cali and Louis M. Weiss},
  journal={Infection and Immunity},
  pages={1374 - 1385}
ABSTRACT The microsporidia are a diverse phylum of obligate intracellular parasites that infect all major animal groups and have been recognized as emerging human pathogens for which few chemotherapeutic options currently exist. These organisms infect every tissue and organ system, causing significant pathology, especially in immune-compromised populations. The microsporidian spore employs a unique infection strategy in which its contents are delivered into a host cell via the polar tube, an… 

Short communication A new vesicular compartment in Encephalitozoon cuniculi

A proteomics-driven effort to find novel components of this organelle in the human-pathogenic species Encephalitozoon cuniculi unexpectedly discovered a protein which localizes to punctate structures consistent with the appearance of relic mitochondria, or mitosomes.

A new vesicular compartment in Encephalitozoon cuniculi.

Microsporidia: Obligate Intracellular Pathogens Within the Fungal Kingdom.

The study of the mechanism of invasion should provide fundamental insights into the biology of these ubiquitous intracellular pathogens that can be integrated into studies aimed at treating or controlling microsporidiosis.

Microsporidiosis in Humans

While the prevalence rate of microsporidiosis in patients with AIDS has fallen in the United States, due to the widespread use of combination antiretroviral therapy (cART), infection continues to occur throughout the world and is still seen in theUnited States in the setting of cART if a low CD4 count persists.

An Ultrastructural Study of the Extruded Polar Tube of Anncaliia algerae (Microsporidia)

This study utilized several types of microscopy, describing and extending the understanding of these structures and their functions, and found that the terminus of the polar tube has a closed tip where the membranes for the terminal sac are located.

Identification and Characterization of Three Spore Wall Proteins of Enterocytozoon Bieneusi

The sequences of three E. bieneusi SWPs were identified from whole genome sequence data, expressed them in Escherichia coli, generated a monoclonal antibody (mAb) against one of them (EbSWP1), and used the mAb in direct immunofluorescence detection of E.Bienesi spores in fecal samples.



Microsporidia: emerging pathogenic protists.

Identification of a New Spore Wall Protein from Encephalitozoon cuniculi

A new spore wall protein (SWP3) that is located in the endospore of Microsporidia is identified and has several potential O-glycosylation sites, and it is possible that it is a mannosylated protein like the major polar tube protein (PTP1).

Developmental Expression of a Tandemly Repeated, Glycine- and Serine-Rich Spore Wall Protein in the Microsporidian Pathogen Encephalitozoon cuniculi

Indirect immunofluorescence and immunoelectron microscopy revealed that SWP1 is differentially expressed during the infection cycle, and Expression ofSWP1 appears to be regulated at the mRNA level, as was shown by reverse transcriptase PCR analysis.

EnP1, a Microsporidian Spore Wall Protein That Enables Spores To Adhere to and Infect Host Cells In Vitro

Data show that given its expression in both the endospore and the exospore, EnP1 is a microsporidian cell wall protein that may function both in a structural capacity and in modulating in vitro host cell adherence and infection.

Developmental Expression of Two Spore Wall Proteins during Maturation of the Microsporidian Encephalitozoon intestinalis

The characterization of two immunogenic SWPs from E. intestinalis will allow the study of exospore structure and function and may lead to the development of useful tools in the diagnosis and treatment of microsporidiosis.

Genome sequence and gene compaction of the eukaryote parasite Encephalitozoon cuniculi

The DNA sequences of the 11 chromosomes of the ∼2.9-megabase (Mb) genome of Encephalitozoon cuniculi are reported and it is hypothesize that microsporidia have retained a mitochondrion-derived organelle.

Detection of microsporidia spore-specific antigens by monoclonal antibodies.

These MAbs may be useful in the diagnosis and speciation of Microsporidia as well in the purification, cloning, and detection of these antigens.