In the last decade, one of the most avidly studied compounds in the central nervous system (CNS) has been the neurotrophin BDNF. Although historically it had been studied in the context of development, where it plays numerous critical roles, more recent studies have shown striking actions on a number of pathways and processes that are critical for normal… CONTINUE READING
Figure 8. Recordings from CA3 pyramidal cells illustrate the intracellular correlate to extracellularly-recorded population spikes induced by repeated mossy fiber stimuli in the presence of increased BDNF. A. Responses to mossy fiber stimuli (paired pulses, 40 msec interstimulus interval, 1 Hz) are recorded from a CA3 pyramidal cell of a slice from a proestrous rat with elevated mossy fiber BDNF. Membrane potential, -72 mV. A longlasting depolarization develops by the 3rd pair of stimuli and increases further by the 6th pair of stimuli. On this depolarization, action potentials are triggered that correspond to the multiple population spikes observed extracellularly, shown in Figure 6. B. Recordings from a CA3 pyramidal cell in a slice from a metestrous rat with relatively low mossy fiber BDNF. Membrane potential, -68 mV. No long-lasting depolarizations or extra action potentials are evoked. The cells from metestrous rats demonstrate similar responses as other CA3 pyramidal cells examined under other conditions when BDNF levels in mossy fibers are relatively low, such as male rats prior to exposure to exogenous BDNF.