Bovine sperm cells viability during incubation with or without exogenous DNA.

  title={Bovine sperm cells viability during incubation with or without exogenous DNA.},
  author={Weber Beringui Feitosa and Marcella Pecora Milazzotto and Renata B Sim{\~o}es and Mariana Rovegno and Alessandra Coralo Nicacio and An{\'i}bal Ballarotti Nascimento and Jos{\'e} Sergio de Arruda Gonçalves and Jose Antonio Visintin and Mayra Elena Ortiz D'{\'A}vila Assumpç{\~a}o},
  volume={17 4},
SummaryThe aim of this study was to assess the effect of exogenous DNA and incubation time on the viability of bovine sperm. Sperm were incubated at a concentration of 5 x 106/ml with or without plasmid pEYFP-NUC. Fluorescent probes, propidium iodide/Hoechst 33342, FITC-PSA and JC-1, were used to assess plasma membrane integrity (PMI), acrosome membrane integrity (AMI) and mitochondrial membrane potential (MMP) respectively at 0, 1, 2, 3 and 4 h of incubation. Exogenous DNA addition did not… CONTINUE READING


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