Bovine papillomavirus type 1 DNA replication: the transcriptional activator E2 acts in vitro as a specificity factor.

@article{BonneAndrea1997BovinePT,
  title={Bovine papillomavirus type 1 DNA replication: the transcriptional activator E2 acts in vitro as a specificity factor.},
  author={Catherine Bonne-Andrea and F Tillier and G D McShan and Van G. Wilson and Philippe Clertant},
  journal={Journal of virology},
  year={1997},
  volume={71 9},
  pages={6805-15}
}
We previously devised cell-free conditions supporting efficient replication of bovine papillomavirus type 1 (BPV1) DNA (C. Bonne-Andréa, S. Santucci, and P. Clertant, J. Virol. 69:3201-3205, 1995): the use of highly active preparations of viral initiator protein E1, together with extract from a particular cell source, allowed the synthesis of complete DNA circles through successive rounds of replication; this occurred in the absence of the viral transcriptional activator E2, required in vivo… CONTINUE READING