Bovine kidney pyruvate dehydrogenase complex. Isolation of the component enzymes after limited proteolysis with papain.

@article{Kresze1980BovineKP,
  title={Bovine kidney pyruvate dehydrogenase complex. Isolation of the component enzymes after limited proteolysis with papain.},
  author={G B Kresze and H Ronft and Barbara Dietl},
  journal={European journal of biochemistry},
  year={1980},
  volume={105 2},
  pages={371-9}
}
1. Bovine kidney pyruvate dehydrogenase multienzyme complex is inactivated rapidly by papain. However, none of the component activities of the complex is destroyed during inactivation of the overall reaction. 2. The core component, lipoate acetyltransferase, is cleaved by papain to give principal fragments with Mr 26,500 and 26,000 (as determined by… CONTINUE READING