Both direct and collagen-mediated signals are required for active vitamin D3-elicited differentiation of human osteoblastic cells: roles of osterix, an osteoblast-related transcription factor.

@article{Maehata2006BothDA,
  title={Both direct and collagen-mediated signals are required for active vitamin D3-elicited differentiation of human osteoblastic cells: roles of osterix, an osteoblast-related transcription factor.},
  author={Yojiro Maehata and Shinji Takamizawa and Shigeyuki Ozawa and Yasumasa Kato and Sadao Sato and Eiro Kubota and Ryu-ichiro Hata},
  journal={Matrix biology : journal of the International Society for Matrix Biology},
  year={2006},
  volume={25 1},
  pages={47-58}
}
In order to investigate the mechanisms by which 1alpha,25(OH)2 vitamin D3 (VD3) stimulates the differentiation of human osteoblasts, we cultured MG-63, which is a human osteoblastic cell line, in the presence or absence of VD3 and/or L-ascorbic acid 2-phosphate (Asc 2-P), a long-acting vitamin C derivative. The cell growth rate was decreased by the presence of VD3 in the culture medium. Type I collagen synthesis and alkaline phosphatase (ALP) activity, which are markers of early stage… CONTINUE READING

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