Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle.

@article{Gassmann2004BorealinAN,
  title={Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle.},
  author={Reto Gassmann and Ana Carvalho and Alexander J. Henzing and Sandrine Ruchaud and Damien F. Hudson and Reiko Honda and Erich A. Nigg and Dietlind L. Gerloff and William C. Earnshaw},
  journal={The Journal of cell biology},
  year={2004},
  volume={166 2},
  pages={
          179-91
        }
}
The chromosomal passenger complex of Aurora B kinase, INCENP, and Survivin has essential regulatory roles at centromeres and the central spindle in mitosis. Here, we describe Borealin, a novel member of the complex. Approximately half of Aurora B in mitotic cells is complexed with INCENP, Borealin, and Survivin; and Borealin binds Survivin and INCENP in vitro. A second complex contains Aurora B and INCENP, but no Borealin or Survivin. Depletion of Borealin by RNA interference delays mitotic… 

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Uncoupling the central spindle-associated function of the chromosomal passenger complex from its role at centromeres.
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The C terminus of Survivin is sufficient for central spindle localization and execution of cytokinesis, but the additional presence of a functional BIR domain is essential for centromere targeting and spindle checkpoint function.
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