Bordetella pertussis adenylate cyclase: purification and characterization of the toxic form of the enzyme.

@article{Rogel1989BordetellaPA,
  title={Bordetella pertussis adenylate cyclase: purification and characterization of the toxic form of the enzyme.},
  author={A Rogel and Joachim E Schultz and R M Brownlie and John Graham Coote and Roger Parton and Emanuel Hanski},
  journal={The EMBO journal},
  year={1989},
  volume={8 9},
  pages={2755-60}
}
Bordetella pertussis produces a calmodulin-sensitive adenylate cyclase (AC) which is an essential virulence factor in mammalian pertussis. Here we report the purification and characterization of the toxic form of the enzyme, which penetrates eukaryotic cells and generates high levels of intracellular cAMP. This form was purified from an extract of B.pertussis strain carrying a recombinant plasmid which over-produced both enzymatic and toxic activities of the enzyme. Western blot analysis of the… CONTINUE READING

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