BootstRatio: A web-based statistical analysis of fold-change in qPCR and RT-qPCR data using resampling methods

@article{Clries2012BootstRatioAW,
  title={BootstRatio: A web-based statistical analysis of fold-change in qPCR and RT-qPCR data using resampling methods},
  author={Ramon Cl{\`e}ries and Jordi Galvez and Meritxell Espino and Josepa Ribes Puig and Virginia Nunes and Miguel L{\'o}pez de Heredia},
  journal={Computers in biology and medicine},
  year={2012},
  volume={42 4},
  pages={438-45}
}
Real-time quantitative polymerase chain reaction (qPCR) is widely used in biomedical sciences quantifying its results through the relative expression (RE) of a target gene versus a reference one. Obtaining significance levels for RE assuming an underlying probability distribution of the data may be difficult to assess. We have developed the web-based application BootstRatio, which tackles the statistical significance of the RE and the probability that RE>1 through resampling methods without any… CONTINUE READING

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