Purification and kinetic study of bone and liver alkaline phosphatase isoenzymes in the dog
Eight clinically healthy male sheepdogs were selected and subjected to experimental radius transaction. Blood samples were taken before and weekly after surgery, and radiographs were taken immediately and weekly after surgery to trace new bone formation. Total protein, total ALP, and bone-specific ALP were measured in serum samples. There was no significant difference in total proteins, but total ALP and bone ALP activity were significantly increased (P < 0.05) after surgery (during bone formation).We found a weak correlation between total ALP activity and BALP activity during the study. The bridging callus was completed in the fourth week of the experiment, and the gap was fully filled with new bone. However, increased levels of total ALP and bone-specific ALP activity were maintained throughout the study and did not reduce at 4 weeks. We concluded that serum activity of bone-specific ALP is a good indicator of bone formation in sheepdogs, and when radiographs inform the completion of bone fracture healing, cellular activity in the healing region is likely to be continuing.