Bone progenitor dysfunction induces myelodysplasia and secondary leukemia

Abstract

CD45 CD31 GFP + osteolineage cells in bone marrow (a) or collagenased bone fragments (b) (n=4) c-e impaired osteogenic differentiation capacity of OCD fl/fl bone marrow stromal cells as shown by reduced CFU-Alk/CFU-F ratio (c) (n=2, performed in quadruplicate), impaired in vitro osteogenic differentiation upon osteogenic induction (d) and decreased osteocalcin gene expression in primary osteolineage cells as shown by RT-PCR on CD45 Lineage CD31 GFP+ cells (n=5) (e) f, altered texture of the bone matrix g, normal trabecular bone volume (BV) and h, increased cortical bone volume as assessed by histomorphometric analysis (n=8)(TV=total volume; BA=bone area, TA total area). i,j reduced osteoblasts (Ob) number per bone surface (Bs) in OCD fl/fl mice as indicated by histomorphometric analysis (n=8) k, the number of osteoclasts (OC) was unaltered in OCD fl/fl mice (n=8). Data are mean ± s.e.m. * p≤0.05, **p≤0.01. CFU-F=colony-forming assay –fibroblast, CFU-ALK=colony forming unit alkaline phosphatase, AP=alkaline phosphatas i

Extracted Key Phrases

010020020102011201220132014201520162017
Citations per Year

460 Citations

Semantic Scholar estimates that this publication has 460 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@inproceedings{Raaijmakers2010BonePD, title={Bone progenitor dysfunction induces myelodysplasia and secondary leukemia}, author={Marc H.G.P. Raaijmakers and Siddhartha Mukherjee and Shangqin Guo and Siyi Zhang and Tatsuya Kobayashi and Jesse A. Schoonmaker and Benjamin L. Ebert and F{\'a}tima Al-Shahrour and Robert Paul Hasserjian and Edward O. Scadden and Zinmar Aung and Marc Matza and Matthias Merkenschlager and Charles P . Lin and Johanna M Rommens and David. T. Scadden}, year={2010} }