Bone Morphogenetic Protein 2 Induces Cyclo-oxygenase 2 in Osteoblasts via a Cbfa1 Binding Site: Role in Effects of Bone Morphogenetic Protein 2 In Vitro and In Vivo

@inproceedings{CHIKAZU2002BoneMP,
  title={Bone Morphogenetic Protein 2 Induces Cyclo-oxygenase 2 in Osteoblasts via a Cbfa1 Binding Site: Role in Effects of Bone Morphogenetic Protein 2 In Vitro and In Vivo},
  author={DAICHI CHIKAZU and Li Xiaodong and HIROSHI KAWAGUCHI and YOKO SAKUMA and OLGA S. VOZNESENSKY and DOUGLAS J. ADAMS and XU MANSHAN and KAZUTO HOSHI and VEDRAN KATAVIC and Harvey R. Herschman and Lawrence G. Raisz and CAROL C. PILBEAM},
  year={2002}
}
We tested the hypothesis that induction of cyclo-oxygenase (COX) 2 mediates some effects of bone morphogenetic protein (BMP) 2 on bone. BMP-2 induced COX-2 mRNA and prostaglandin (PG) production in cultured osteoblasts. BMP-2 increased luciferase activity in calvarial osteoblasts from mice transgenic for a COX-2 promoter-luciferase reporter construct (Pluc) and in MC3T3-E1 cells transfected with Pluc. Deletion analysis identified the 300/ 213-bp region of the COX-2 promoter as necessary for BMP… CONTINUE READING