Osteoporosis is a metabolic disorder of bone that is characterized by low bone mineral density and deterioration of micro-architecture of the bone tissue leading to increased skeletal fragility and increased fracture risk. The increased risk of osteoporosis in postmenopausal women is explained by biochemical markers of bone turnover and bone mineral density which help in early identification of women who are at increased risk of fractures. The objective of the study was to analyze biochemical markers of bone turnover and bone mineral density measurements by Bone densitometer (using T-score) among postmenopausal women in comparison to premenopausal women. A cross sectional prospective study was carried, including 40 healthy premenopausal women of 25-45 years age and 40 healthy postmenopausal women of 46-65 years age. Biochemical markers of bone turnover (Serum calcium, Phosphorus, Alkaline phosphatase) and bone mineral density were assessed among all the participants. An unpaired student T-test was used to test differences between groups. Bone formation markers, total calcium, phosphorus were significantly decreased (p < 0.001), serum alkaline phosphatase was significantly increased (p<0.001) in postmenopausal women when compared to premenopausal women. Bone mineral density was significantly decreased in postmenopausal women than premenopausal women. This study suggests simple common biochemical markers of bone turnover and bone mineral density measurement can be used to assess the osteoporosis in postmenopausal women. We recommend that postmenopausal women should be screened for osteoporotic fracture risk which might be an important strategy in the management of postmenopausal osteoporotic fracture risk.