Bone‐Staining Technique for Fetal Rat Specimens without Skinning and Removing Adipose Tissue

  title={Bone‐Staining Technique for Fetal Rat Specimens without Skinning and Removing Adipose Tissue},
  author={Satoshi Kawamura and Atsuko Hirohashi and Terushige Kato and Mineo Yasuda},
  journal={Congenital Anomalies},
Abstract: A rapid procedure was developed for staining fetal rat specimens. Full‐term rat fetuses were fixed in 99% ethanol and only thoracoabdominal organs were removed. Without skinning and removing adipose tissue and eye balls, the specimens were placed in 85% ethanol containing 1.5% KOH and 0.0015% alizarin red S for 5 days. The specimens were macerated in 1% aqueous KOH and cleared in 50% glycerin solution. The skeleton was visible through surrounding tissue containing adipose tissue which… Expand
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A novel approach to study the development and mineralization of avian scleral ossicles during embryogenesis was presented and validated by studying two different species, both important models for avian developmental research. Expand
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Developmental toxicity of concanavalin A in rats: association with restricted migration of neural crest cells.
  • A. Nishida, T. Kobayashi, F. Ariyuki
  • Biology, Medicine
  • Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
  • 1996
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A Note on the Staining of the Skeleton of Cleared Specimens with Alizarin Red S
A selective, progressive method for staining the skeleton in cleared specimens, developed with rat material.Fix in 95% alcohol for at least 48 to 96 hrs. Even longer fixation is desirable. Then placeExpand
A rapid pro - Technol
  • 1981
Refinements in rapid clearing technic in the KOH-alizarin red S cedure for routine double staining of cartilage method for fetal bone
  • A rapid pro- Technol.,
  • 1964
A note on the staining of the and bone in fetal and adult animals
  • Stain skeleton of cleared specimens with alizarin red S . Stain Technol .
  • 1926
A note on the staining of the and bone in fetal and adult animals. Stain skeleton of cleared specimens with alizarin red S
  • Stain Technol
  • 1926