Bone‐Staining Technique for Fetal Rat Specimens without Skinning and Removing Adipose Tissue

@article{Kawamura1990BoneStainingTF,
  title={Bone‐Staining Technique for Fetal Rat Specimens without Skinning and Removing Adipose Tissue},
  author={Satoshi Kawamura and Atsuko Hirohashi and Terushige Kato and Mineo Yasuda},
  journal={Congenital Anomalies},
  year={1990},
  volume={30}
}
Abstract: A rapid procedure was developed for staining fetal rat specimens. Full‐term rat fetuses were fixed in 99% ethanol and only thoracoabdominal organs were removed. Without skinning and removing adipose tissue and eye balls, the specimens were placed in 85% ethanol containing 1.5% KOH and 0.0015% alizarin red S for 5 days. The specimens were macerated in 1% aqueous KOH and cleared in 50% glycerin solution. The skeleton was visible through surrounding tissue containing adipose tissue which… Expand
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