Bombesin stimulates the motility of human prostate‐carcinoma cells through tyrosine phosphorylation of focal adhesion kinase and of integrin‐associated proteins

@article{Aprikian1997BombesinST,
  title={Bombesin stimulates the motility of human prostate‐carcinoma cells through tyrosine phosphorylation of focal adhesion kinase and of integrin‐associated proteins},
  author={A. Aprikian and L. Tremblay and K. Han and S. Chevalier},
  journal={International Journal of Cancer},
  year={1997},
  volume={72}
}
Bombesin‐like peptides, including the mammalian homologue gastrin‐releasing peptides, are highly expressed and secreted by neuroendocrine cells in prostate carcinoma (PCa) tissues and are likely to be related to the progression of this disease. In the present study, we show that bombesin enhances the migration of androgen‐independent PCa cells (PC‐3) in vitro, while not affecting their adhesion to extracellular matrix proteins. The bombesin‐increased motility of PC‐3 cells occurs through its… Expand
Activation of extracellular signal-regulated kinase mediates bombesin-induced mitogenic responses in prostate cancer cells.
TLDR
Data suggest bombesin may act as a mitogen in prostate cancer by activating MAP kinase pathway via EGFR transactivation through increased extracellular regulated kinase (ERK) phosphorylation and epidermal growth factor (EGF) receptor transactivation in prostatecancer cells, which express functional gastrin-releasing peptide receptor. Expand
Focal adhesion kinase is required for bombesin-induced prostate cancer cell motility
TLDR
Observations point towards a critical role for FAK in the action of BN on PC-3 cell motility in prostate cancer. Expand
Bombesin stimulates invasion and migration of Isreco1 colon carcinoma cells in a Rho-dependent manner.
TLDR
The results show that the neuropeptide bombesin is able to modulate invasiveness of Isreco1 colorectal carcinoma cells in vitro through a Rho-dependent pathway, leading to an increase in cell locomotion without a significant effect on tumor-cell associated proteolytic activity. Expand
Neuropeptide-stimulated cell migration in prostate cancer cells is mediated by RhoA kinase signaling and inhibited by neutral endopeptidase
TLDR
A contiguous signaling pathway from the bombesin receptor to ROCK in PC cells is established, and NEP is implicate as a major regulator of neuropeptide-stimulated RhoA in these cells. Expand
Bombesin modulates the association of Src with a nuclear 110-kd protein expressed in dividing prostate cells.
TLDR
Nuclear p110 protein, expressed in dividing prostate epithelial cells and in human prostate cancer cells and tissues, could thus be a downstream mediator of bombesin-signaling pathways, acting via its association with Src. Expand
Neutral endopeptidase inhibits prostate cancer cell migration by blocking focal adhesion kinase signaling.
TLDR
It is demonstrated that NEP can inhibit FAK phosphorylation on tyrosine and PC cell migration through multiple pathways and suggest that cell migration which contributes to invasion and metastases in PC cells can be regulated by NEP. Expand
Bombesin induces cyclooxygenase-2 expression through the activation of the nuclear factor of activated T cells and enhances cell migration in Caco-2 colon carcinoma cells
TLDR
It is shown that bombesin (BBS), a GRP homolog, stimulates the expression of Cox-2 mRNA and protein in human colon adenocarcinoma Caco-2 cells, resulting in enhanced release of prostaglandin E2, the first evidence for the involvement of the Ca2+/Cn/NFAT pathway in BBS-mediated induction of genes involved in colon carcinoma invasiveness such as Cox- 2. Expand
Bombesin stimulates adhesion, spreading, lamellipodia formation, and proliferation in the human colon carcinoma Isreco1 cell line.
TLDR
Results clearly indicate that bombesin exerts morphological, adhesive, and proliferative effects on Isreco1 cells, suggesting that expression of theBombesin/GRP-R may contribute to the malignant properties of colon carcinoma cells. Expand
Bombesin stimulates nuclear factor kappa B activation and expression of proangiogenic factors in prostate cancer cells.
TLDR
A role for BBS and GRP-R is demonstrated in the NF kappa B-dependent up-regulation of proangiogenic gene expression, and a possible molecular mechanism linking NE differentiation and the increased metastatic potential of androgen-insensitive prostate cancers is suggested. Expand
Novel Coactivators for Androgen Receptor
TLDR
Paxillin, a protein that responds to the metastatic state of prostate cancer cells, can directly influence androgen receptor transactivation through its carboxyl-terminal LIM domain. Expand
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Results show that an increase in FAK mRNA and protein, as well as pp125FAK activation and association with signalling proteins, correlates with progression and invasion in human PCa tissues and cells. Expand
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The data indicate that bombesin is a potent inducer of signal transduction via GRP-R receptors in androgen-insensitive PC-3 and DU-145 prostate cancer cells, and suggests that theBombesin/GRP family of neuropeptides may play a regulatory role in the biology of androgens-independent prostate cancer. Expand
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TLDR
Jasplakinolide and CyE inhibited bombesin-stimulated phosphorylation of FAK and inhibited PC-3 cell growth, suggesting actin-disrupting agents block FAK signal transduction, which may be critical to their antitumor activity in prostate carcinoma. Expand
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The results suggest that certain neuroendocrine peptides can increase the invasive potential of prostatic carcinoma cells and may thereby contribute to the rapid progression and aggressive clinical course of prostate tumors containing neuro endocrine elements. Expand
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