Bohr-effect and pH-dependence of electron spin resonance spectra of a cobalt-substituted monomeric insect haemoglobin

@article{Gersonde1982BohreffectAP,
  title={Bohr-effect and pH-dependence of electron spin resonance spectra of a cobalt-substituted monomeric insect haemoglobin},
  author={Klaus Gersonde and Hans Twilfer and M J Overkamp},
  journal={Biophysics of structure and mechanism},
  year={1982},
  volume={8},
  pages={189-211}
}
The monomeric haemoglobin IV from Chironomus thummi thummi (CTT IV) exhibits an alkaline Bohr-effect and therefore it is an allosteric protein. By substitution of the haem iron for cobalt the O2 half-saturation pressure, measured at 25‡ C, increases 250-fold. The Bohr-effect is not affected by the replacement of the central atom. The parameters of the Bohr-effect of cobalt CTT IV for 25‡ C are: inflection point of the Bohr-effect curve at pH 7.1, number of Bohr protons -δlog p1/2 (O2)/gDpH=0.36… CONTINUE READING
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