Boar acrosin, I: modified isolation procedure, active-site titration and evidence for the presence of sialic acid.

Abstract

Boar acrosin was isolated to electrophoretic homogeneity by modification of the method described by us previously. Titration with p-nitrophenyl p-guanidinobenzoate revealed a molar activity of 8900 toward BzArgOEt and of 780 towards BzArgNHNp. Treatment with neuraminidase led to an altered electrophoretic mobility, indicating that acrosin is… (More)

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