Blunt injury augments interleukin-6 but not tumor necrosis factor in isolated, perfused rat hindlimbs.

@article{Cipolle1994BluntIA,
  title={Blunt injury augments interleukin-6 but not tumor necrosis factor in isolated, perfused rat hindlimbs.},
  author={Mark D. Cipolle and Michael D. Pasquale and J Shearer and Marthajane Caldwell and Frank B. Cerra},
  journal={The Journal of trauma},
  year={1994},
  volume={37 1},
  pages={
          91-8; discussion 98-9
        }
}
An isolated, perfused hindlimb model in rats was used to examine the immediate inflammatory response after blunt tissue injury. A femur-fracture degloving model was used in isolated rat hindlimbs perfused with a modified Kreb's buffer (pH 7.4) containing albumin, washed human red blood cells (RBCs), amino acids, and glucose at 37 degrees C. Arterial and venous perfusate was sampled at 5, 20, and 80 minutes of perfusion. Initial experiments were conducted in perfusate void of white blood cells… CONTINUE READING