Blotting from PhastGel to membranes by ultrasound.

  • Joseph Kost
  • Published 2009 in Methods in molecular biology


Ultrasound-based approach for enhanced protein blotting is proposed. Three minutes of ultrasound exposure (1 MHz, 2.5 W/cm(2)) was sufficient for a clear transfer of proteins from a polyacrylamide gel (PhastGel) to nitrocellulose or Nylon 66 Biotrans membrane. The proteins evaluated were prestained sodium dodecyl sulfate-polyacrylamide standards (18,500-106… (More)
DOI: 10.1007/978-1-59745-542-8_19