Blockface histology with optical coherence tomography: A comparison with Nissl staining

@article{Magnain2014BlockfaceHW,
  title={Blockface histology with optical coherence tomography: A comparison with Nissl staining},
  author={Caroline Magnain and Jean Augustinack and Martin Reuter and Christian Wachinger and Matthew P. Frosch and Timothy Ragan and Taner Akkin and Van J. Wedeen and David A. Boas and Bruce Fischl},
  journal={NeuroImage},
  year={2014},
  volume={84},
  pages={524-33}
}
Spectral domain optical coherence tomography (SD-OCT) is a high resolution imaging technique that generates excellent contrast based on intrinsic optical properties of the tissue, such as neurons and fibers. The SD-OCT data acquisition is performed directly on the tissue block, diminishing the need for cutting, mounting and staining. We utilized SD-OCT to visualize the laminar structure of the isocortex and compared cortical cytoarchitecture with the gold standard Nissl staining, both… CONTINUE READING