Blockade of the PD-1/PD-L1 axis augments lysis of AML cells by the CD33/CD3 BiTE antibody construct AMG 330: reversing a T-cell-induced immune escape mechanism

@article{Krupka2016BlockadeOT,
  title={Blockade of the PD-1/PD-L1 axis augments lysis of AML cells by the CD33/CD3 BiTE antibody construct AMG 330: reversing a T-cell-induced immune escape mechanism},
  author={Christina Krupka and Peter Kufer and Roman Kischel and Gerhard Zugmaier and Felix Lichtenegger and Thomas K{\"o}hnke and Binje Vick and Irmela Jeremias and Klaus H Metzeler and Torben Altmann and Stephanie Schneider and Michael Fiegl and Karsten Spiekermann and Patrick A Bauerle and Wolfgang Hiddemann and Gert Riethmueller and Marion Subklewe},
  journal={Leukemia},
  year={2016},
  volume={30},
  pages={484-491}
}
Bispecific T-cell engagers (BiTEs) are very effective in recruiting and activating T cells. We tested the cytotoxicity of the CD33/CD3 BiTE antibody construct AMG 330 on primary acute myeloid leukemia (AML) cells ex vivo and characterized parameters contributing to antileukemic cytolytic activity. The E:T ratio and the CD33 expression level significantly influenced lysis kinetics in long-term cultures of primary AML cells (n=38). AMG 330 induced T-cell-mediated proinflammatory conditions… CONTINUE READING