Blockade of the Noradrenaline Carrier Increases Extracellular Dopamine Concentrations in the Prefrontal Cortex: Evidence that Dopamine Is Taken up In Vivo by Noradrenergic Terminals

  title={Blockade of the Noradrenaline Carrier Increases Extracellular Dopamine Concentrations in the Prefrontal Cortex: Evidence that Dopamine Is Taken up In Vivo by Noradrenergic Terminals},
  author={Ezio Carboni and Gianluigi Tanda and Roberto Frau and Gaetano di Chiara},
  journal={Journal of Neurochemistry},
Abstract: The effect of systemic administration of desmethylimipramine (DMI) and oxaproptiline (OXA), two inhibitors of the noradrenaline (NA) reuptake carrier, on the in vivo extracellular concentrations of dopamine (DA) was studied by transcerebral dialysis in the prefrontal cortex and in the dorsal caudate of freely moving rats. In the NA‐rich prefrontal cortex, either drug increased extracellular DA concentrations whereas in the dorsal caudate neither was effective. Haloperidol increased… 

Co-release of noradrenaline and dopamine from noradrenergic neurons in the cerebral cortex induced by clozapine, the prototype atypical antipsychotic

The results suggest that clozapine, by inhibiting α2-adrenoceptors, co-releases DA and NA from noradrenergic terminals in the occipital cortex and that the same mechanism might be responsible for the concomitant increase of the two monoamines in the mPFC.

Evidence that Extracellular Concentrations of Dopamine Are Regulated by Noradrenergic Neurons in the Frontal Cortex of Rats

The hypothesis of an important regulation of the extracellular concentrations of DA in the frontal cortex by noradrenergic terminals is supported.

Contribution of Blockade of the Noradrenaline Carrier to the Increase of Extracellular Dopamine in the Rat Prefrontal Cortex by Amphetamine and Cocaine

It is suggested that amphetamine and cocaine increase extracellular DA in the PFCX largely through the blockade of the NA carrier, and direct evidence for this hypothesis was provided by the observation that, when theNA carrier was blocked by reverse dialysis of the P FCX with desipramine, cocaine and GBR 12909 lost their differences in the ability to increase extracllular DA.

Regulation of Extracellular Dopamine by the Norepinephrine Transporter

It is suggested that norepinephrine terminals regulate extracellular dopamine concentrations in the medial prefrontal cortex and to a lesser extent in the nucleus accumbens shell through the uptake of dopamine by the norpinephrine transporter.

Alpha2‐adrenoceptor mediated co‐release of dopamine and noradrenaline from noradrenergic neurons in the cerebral cortex

The hypothesis that most extracellular dopamine in the cortex is co‐released with NA from noradrenergic terminals, such co‐release being primarily controlled by α2‐adrenoceptors is supported.

Noradrenergic Source of Dopamine Assessed by Microdialysis in the Medial Prefrontal Cortex

The results indicate that DA released in the mPFC by dopaminergic terminals is not detected by microdialysis unless DA clearance from extracellular space is inactivated, supporting the hypothesis that noradrenergic terminals are the main source of DA measured by micro Dialysis in themPFC during physiologically relevant activities.

On the origin of cortical dopamine: is it a co-transmitter in noradrenergic neurons?

Evidence points to a common origin for NA and DA in the cerebral cortex and suggests the possible utility of noradrenergic system modulation as a target for drugs with potential clinical efficacy on cognitive functions.

Local Influence of Endogenous Norepinephrine on Extracellular Dopamine in Rat Medial Prefrontal Cortex

The hypothesis that NE terminals in medial prefrontal cortex regulate extracellular DA in this region is supported by mechanisms involving an action of NE on receptors that regulate DA release and/or transport of DA into noradrenergic terminals (heterotransporter regulation).



Evidence that [3H]Dopamine Is Taken Up and Released from Nondopaminergic Nerve Terminals in the Rat Substantia Nigra In Vitro

In tissue preparations from substantia nigra, the in vitro uptake and release of [3H]dopamine may not reflect dendritic dopamine function alone, but may involve other neuronal processes, particularly 5‐HT neurones.

Dopamine release and metabolism in awake rats after systemic neuroleptics as studied by trans-striatal dialysis.

  • A. ImperatoG. Di Chiara
  • Biology, Medicine
    The Journal of neuroscience : the official journal of the Society for Neuroscience
  • 1985
The method of trans-striatal dialysis has been applied here to the study of the release and metabolism of dopamine (DA) in the awake rat and indicates that stimulation of DA release by neuroleptics is strictly dependent upon stimulation ofDA firing and that different mechanisms underline their effects on DA release and on DA metabolism.

Structure‐activity relations for the inhibition of catecholamine uptake into synaptosomes from noradrenaline and dopaminergic neurones in rat brain homogenates

  • A. Horn
  • Biology, Chemistry
    British journal of pharmacology
  • 1973
It was found that, in general, the noradrenaline uptake process was more sensitive to structural changes, both positive and negative, than the dopamine system.

In-vivo brain dialysis of neurotransmitters.

  • G. Di Chiara
  • Biology, Psychology
    Trends in pharmacological sciences
  • 1990

Trans-striatal dialysis coupled to reverse phase high performance liquid chromatography with electrochemical detection: a new method for the study of the in vivo release of endogenous dopamine and metabolites

  • A. ImperatoG. Di Chiara
  • Biology, Chemistry
    The Journal of neuroscience : the official journal of the Society for Neuroscience
  • 1984
The biochemical and histological results obtained indicate that the method is suitable to estimate in the rat the changes in the release of endogenous DA and its metabolites which take place in vivo under administration of centrally acting drug.