Bleomycin-specific fragmentation of double-stranded DNA.

@article{Lloyd1978BleomycinspecificFO,
  title={Bleomycin-specific fragmentation of double-stranded DNA.},
  author={R. Stephen Lloyd and C. W. Haidle and Donald L. Robberson},
  journal={Biochemistry},
  year={1978},
  volume={17 10},
  pages={1890-6}
}
Brief exposure of covalently closed circular duplex PM2 DNA to low concentrations of the clinical bleomycin mixture (Blenoxane) resulted in specific fragmentation of the genome that does not depend on the presence of superhelical turns. The double-strand breaks are in fact produced at several discrete sites on the PM2 genome but frequently occurring near the HpaII restriction endonuclease cleavage site. Initial rates of formation of nicked circular and linear duplex PM2 DNAs are reduced to… CONTINUE READING

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