Bleomycin resistance: a new dominant selectable marker for plant cell transformation

  title={Bleomycin resistance: a new dominant selectable marker for plant cell transformation},
  author={Jacques Hille and Frank W. S. M. Verheggen and Peter W. Roelvink and Henk J. Franssen and Ab van Kammen and Pim Zabel},
  journal={Plant Molecular Biology},
Plant cells are sensitive to the antibiotic bleomycin, a DNA damaging glycopeptide. A bleomycin resistance determinant, located on transposon Tn5 and functional in bacteria, has been cloned in a plant expression vector and introduced into Nicotiana plumbaginifolia using Agrobacterium tumefaciens. The expression of this determinant in plant cells confers resistance to bleomycin and allows selection of transformed plant cells. 


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