Bleomycin-DNA interactions: fluorescence and proton magnetic resonance studies.

@article{Chien1977BleomycinDNAIF,
  title={Bleomycin-DNA interactions: fluorescence and proton magnetic resonance studies.},
  author={M M Chien and Arthur P. Grollman and Susan Band Horwitz},
  journal={Biochemistry},
  year={1977},
  volume={16 16},
  pages={2641-7}
}
The interaction of bleomycin A2 with DNA has been examined by fluorescence spectroscopy and proton magnetic resonance techniques. Fluorescence bands observed at 353 and 405 nm in the spectrum of bleomycin were assigned to the bithiazole and 4-aminopyrimidine rings, respectively. Quenching of bithiazole fluorescence by DNA was used to determine apparent equilibrium constants for the complex which, in 2.5 mM tris(hydroxymethyl)aminomethane buffer, pH 8.4, are 1.2 X 10(5) M-1 for bleomycin and 1.4… CONTINUE READING