Blastomeres from somatic cell nuclear transfer embryos are not allocated randomly in chimeric blastocysts.

@article{Wells2000BlastomeresFS,
  title={Blastomeres from somatic cell nuclear transfer embryos are not allocated randomly in chimeric blastocysts.},
  author={Karen Wells and Anne M. Powell},
  journal={Cloning},
  year={2000},
  volume={2 1},
  pages={9-22}
}
A marker has been developed to allow detection of blastomeres that originate from embryos produced by nuclear transfer (NT) of genetically engineered fetal fibroblasts. A plasmid (phEFnGFP) was constructed with a G418 resistance cassette for selection in fibroblasts and a nuclear localized green fluorescent protein (nGFP) expression cassette that expresses in every cell of day-6, -7, and -8 bovine embryos. This construct was utilized to follow the blastomere distribution in aggregation chimeras… CONTINUE READING
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