Blastomere fixation techniques and risk of misdiagnosis for preimplantation genetic diagnosis of aneuploidy.

@article{Velilla2002BlastomereFT,
  title={Blastomere fixation techniques and risk of misdiagnosis for preimplantation genetic diagnosis of aneuploidy.},
  author={Esther Velilla and Tom{\'a}s Escudero and Santiago Munn{\'e}},
  journal={Reproductive biomedicine online},
  year={2002},
  volume={4 3},
  pages={
          210-7
        }
}
One of the most critical steps in preimplantation genetic diagnosis (PGD) studies is the fixation required to obtain good fluorescence in-situ hybridization (FISH) nuclear quality without losing any of the cells analysed. Different fixation techniques have been described. The aim of this study was to compare three fixation methods (1, acetic acid/methanol; 2, Tween 20; 3, Tween 20 and acetic acid/methanol) based on number of cells lost after fixation, average rate of informative cells, rate of… CONTINUE READING

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