Bivalency and epitope specificity of a high-affinity IgG3 monoclonal antibody to the Streptococcus group A carbohydrate antigen. Molecular modeling of a Fv fragment.

@article{Pitner2000BivalencyAE,
  title={Bivalency and epitope specificity of a high-affinity IgG3 monoclonal antibody to the Streptococcus group A carbohydrate antigen. Molecular modeling of a Fv fragment.},
  author={J. Bruce Pitner and Wayne F. Beyer and T M Venetta and Colleen M. Nycz and Martin J. Mitchell and Shannon L. Harris and J. R. Marino-albernas and France-Isabelle Auzanneau and Farzin Forooghian and B. Mario Pinto},
  journal={Carbohydrate research},
  year={2000},
  volume={324 1},
  pages={
          17-29
        }
}
Doubly Branched Hexasaccharide Epitope on the Cell Wall Polysaccharide of Group A Streptococci Recognized by Human and Rabbit Antisera
TLDR
Inhibition studies suggested that the group A carbohydrate, unlike the group B streptococcal polysaccharide, does not contain the disaccharide α-l-Rhap(1→2)α-l’Rhap motif at its nonreducing chain terminus, stressing the importance of mapping the determinant specificities of these two important strePTococcal subcapsular group poly Saccharides.
Immunological Evidence for Peptide-Carbohydrate Mimicry with a Group A Streptococcus Polysaccharide-Mimetic Peptide
TLDR
Data demonstrate conclusively the cross-reactivity of the immune responses and support the hypothesis of antigenic mimicry of the GAS-CWPS by the hexapeptide DRPVPY.
Thermodynamics and Density of Binding of a Panel of Antibodies to High-Molecular-Weight Capsular Polysaccharides
TLDR
Results suggest that steric interactions between antibody molecules are a major influence on the values of N of high-affinity MAbs to capsular PSs.
Characterization of Functional Oligosaccharide Mimics of the Shigella flexneri Serotype 2a O-Antigen: Implications for the Development of a Chemically Defined Glycoconjugate Vaccine1
TLDR
It was demonstrated that induction of an efficient serotype 2a-specific anti-O-Ag Ab response was dependent on the length of the oligosaccharide sequence, which was a potential candidate for further development of a chemically defined glycoconjugate vaccine against S. flexneri 2a infection.
Evaluation of glycoconjugate antigens as vaccine candidates against group A Streptococcus and human immunodeficiency virus infections
TLDR
The development of neoglycoconjugates displaying clustered HIV-1 related oligomannose carbohydrates and their immunogenic properties are described to evaluate the potential to exploit a synthetic design approach to GAS vaccine development and compares the efficiency of synthetic antigens with the long isolated GAS polysaccharide.
Toward a Better Understanding of the Basis of the Molecular Mimicry of Polysaccharide Antigens by Peptides
TLDR
Using NMR-derived pentasaccharide and peptide conformations coupled to STD information, models of antigen-antibody interaction were obtained and only one model was found compatible with experimental data when large O-SP fragments were docked into one of the mIgA-binding sites.
Conformational Studies of the O-specific Polysaccharide of Shigella flexneri 5a and of Four Related Synthetic Pentasaccharide Fragments Using NMR and Molecular Modeling*
TLDR
The NMR study, based on the analysis of 1H and 13C chemical shifts, the evaluation of inter-residue distances, and the measurement of one- and three-bond heteronuclear coupling constants, showed that the conformation of one of the four pentasaccharides is similar to that of the native O-SP in solution.
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TLDR
It appears that anti-GAC antibodies are composed of H chains bearing a few VH regions pairing with a few L chains, similar to previous estimates of clonal repertoires of approximately 200.
Idiotope structure and genetic diversity in anti-streptococcal group A carbohydrate antibodies.
Three cross-reactive idiotopes(Id), termed IdX, IdI-1, and Id5, that are present on free L chains from murine anti-group A streptococcal carbohydrate antibodies have been mapped; these Id distinguish
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TLDR
The contributions made to immunology and to bacteriology by the system of antibodies of restricted heterogeneity are pointed out.
Transferred nuclear Overhauser enhancement experiments show that the monoclonal antibody strep 9 selects a local minimum conformation of a Streptococcus group A trisaccharide-hapten.
TLDR
Improved insights about the conformational preferences of the glycosidic linkages of the trisaccharide ligand showed that the free ligand populates various conformations in aqueous solution, thus displaying relatively flexible behavior.
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TLDR
The binding of several oligosaccharide haptens by a monoclonal antibody, Se155-4, specific for Salmonella serogroup B O-antigen was studied by titration microcalorimetry, obviating the requirement for precise values of antibody absorption coefficients and minimizing effects due to partially inactive antibody preparations.
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