Bistability of an In Vitro Synthetic Autoregulatory Switch

@inproceedings{Subsoontorn2011BistabilityOA,
  title={Bistability of an In Vitro Synthetic Autoregulatory Switch},
  author={P. Subsoontorn and J. Kim and E. Winfree},
  year={2011}
}
  • P. Subsoontorn, J. Kim, E. Winfree
  • Published 2011
  • Biology
  • The construction of synthetic biochemical circuits is an essential step for developing quantitative understanding of information processing in natural organisms. Here, we report construction and analysis of an in vitro circuit with positive autoregulation that consists of just four synthetic DNA strands and three enzymes, bacteriophage T7 RNA polymerase, Escherichia coli ribonuclease (RNase) H, and RNase R. The modularity of the DNA switch template allowed a rational design of a synthetic DNA… CONTINUE READING
    8 Citations

    Figures from this paper

    Synthetic in vitro transcriptional oscillators
    • 232
    • PDF
    Timing molecular motion and production with a synthetic transcriptional clock
    • 170
    • PDF
    Analysis, design, and in vitro implementation of robust biochemical networks
    • 7
    • PDF
    Synthetic in vitro transcription circuits
    • 6
    Synthetic in vitro circuits.
    • 51
    • PDF
    Epigenetic information in the cell : a potential avenue for biocompatible computing

    References

    SHOWING 1-10 OF 36 REFERENCES
    Construction of an in vitro bistable circuit from synthetic transcriptional switches
    • 249
    • PDF
    A modular and extensible RNA-based gene-regulatory platform for engineering cellular function
    • M. Win, C. Smolke
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences
    • 2007
    • 355
    • PDF
    A synthetic oscillatory network of transcriptional regulators
    • 3,913
    • PDF
    Engineered riboregulators enable post-transcriptional control of gene expression
    • 477
    • PDF
    Engineering Entropy-Driven Reactions and Networks Catalyzed by DNA
    • 704
    • PDF
    Development of Genetic Circuitry Exhibiting Toggle Switch or Oscillatory Behavior in Escherichia coli
    • 677
    • PDF
    A fast, robust, and tunable synthetic gene oscillator
    • 1,003
    • PDF
    Programmable ligand-controlled riboregulators of eukaryotic gene expression
    • 340
    • PDF
    Neural Network Computation by In Vitro Transcriptional Circuits
    • 73
    • PDF
    Prediction and measurement of an autoregulatory genetic module
    • 456
    • PDF