Bistability of an In Vitro Synthetic Autoregulatory Switch

  title={Bistability of an In Vitro Synthetic Autoregulatory Switch},
  author={P. Subsoontorn and J. Kim and E. Winfree},
  • P. Subsoontorn, J. Kim, E. Winfree
  • Published 2011
  • Biology
  • The construction of synthetic biochemical circuits is an essential step for developing quantitative understanding of information processing in natural organisms. Here, we report construction and analysis of an in vitro circuit with positive autoregulation that consists of just four synthetic DNA strands and three enzymes, bacteriophage T7 RNA polymerase, Escherichia coli ribonuclease (RNase) H, and RNase R. The modularity of the DNA switch template allowed a rational design of a synthetic DNA… CONTINUE READING
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