Bisphosphonate mRNA cap analog attached to Sepharose for affinity chromatography of decapping enzymes.

@article{Szczepaniak2008BisphosphonateMC,
  title={Bisphosphonate mRNA cap analog attached to Sepharose for affinity chromatography of decapping enzymes.},
  author={Sylwia Anna Szczepaniak and Jacek Jemielity and Joanna Żuberek and Joanna Kufel and Edward Darzynkiewicz},
  journal={Nucleic acids symposium series},
  year={2008},
  volume={52},
  pages={295-6}
}
m(7)GTP-Sepharose is routinely used for cap binding protein isolation. Here we present the synthesis of a new affinity resin containing a mononucleotide cap analog resistant to hydrolysis by DcpS. The resin has been designed in order to identify and purify Arabidopsis thaliana DcpS and other pyrophosphatases. The binding efficiency of the new resin to eIF4E… CONTINUE READING