Biphasic regulation of ENaC by TGF-{alpha} and EGF in renal epithelial cells.

  title={Biphasic regulation of ENaC by TGF-\{alpha\} and EGF in renal epithelial cells.},
  author={Lian Jing Liu and Billie Jeanne Duke and Bela Malik and Qiang Yue and Douglas C. Eaton},
  journal={American journal of physiology. Renal physiology},
  volume={296 6},
The epithelial sodium channel (ENaC) is regulated by epidermal growth factor (EGF). We investigate whether ENaC is regulated by another EGF receptor (EGFR) ligand, transforming growth factor-alpha (TGF-alpha). We show that chronic (24 h) treatment with TGF-alpha inhibits ENaC in Xenopus laevis kidney cells 20 times more strongly than EGF. By using single-channel measurements, we show that TGF-alpha significantly reduces the number of ENaC per patch. The open probability (P(o)) is unchanged by… CONTINUE READING