Biphasic extracellular proteolytic enzyme activity in benthic water and sediment in the northwestern mediterranean Sea

@article{Tholosan1999BiphasicEP,
  title={Biphasic extracellular proteolytic enzyme activity in benthic water and sediment in the northwestern mediterranean Sea},
  author={Tholosan and Lamy and Garcin and Polychronaki and Bianchi},
  journal={Applied and environmental microbiology},
  year={1999},
  volume={65 4},
  pages={1619-26}
}
In this study, we used the fact that bacteria are able to cleave a fluorogenic substrate analog (L-leucine-7-amido-4-methylcoumarin) to determine the maximal ectoproteolytic activities (Vm) and affinities (Km) of natural benthic microbial communities by the multiconcentration kinetic method. This investigation was performed during the winter and summer of… CONTINUE READING