Biotransformation of mulberroside A from Morus alba results in enhancement of tyrosinase inhibition

  title={Biotransformation of mulberroside A from Morus alba results in enhancement of tyrosinase inhibition},
  author={Jeong-Keun Kim and Mijin Kim and Ssang-Goo Cho and Myung Kyoo Kim and Suhng-wook Kim and Young-Hee Lim},
  journal={Journal of Industrial Microbiology \& Biotechnology},
Mulberroside A, a glycosylated stilbene, was isolated and identified from the ethanol extract of the roots of Morus alba. Oxyresveratrol, the aglycone of mulberroside A, was produced by enzymatic hydrolysis of mulberroside A using the commercial enzyme Pectinex®. Mulberroside A and oxyresveratrol showed inhibitory activity against mushroom tyrosinase with an IC50 of 53.6 and 0.49 μM, respectively. The tyrosinase inhibitory activity of oxyresveratrol was thus approximately 110-fold higher than… 

An Efficient Preparation of Mulberroside A from the Branch Bark of Mulberry and Its Effect on the Inhibition of Tyrosinase Activity

The enzymatic analysis showed that MA inhibited the generation of dopachrome by affecting the activities of monophenolase and diphenolases of tyrosinase in vitro, providing important information concerning the inhibitory mechanism of MA on melanin synthesis, which is widely used in whitening cosmetics.

Enhancement of the Tyrosinase Inhibitory Activity of Mori Cortex Radicis Extract by Biotransformation Using Leuconostoc paramesenteroides PR

The tyrosinase inhibitory activity of the crude extract of M CR was increased approximately 6.5-fold by biotransformation using LP, and the IC(50) value of the transformed MCR was 3.7 µg/mL.

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The results may indicate that immobilization slowed the growth rate and stimulated mulberroside A release of M. alba cell cultures, and the combination between immobilization and elicitation showed a synergistic effect to enhance the production of mulber roside A.

Inhibitory effect of mulberroside A and its derivatives on melanogenesis induced by ultraviolet B irradiation.

Evaluation of the inhibition of mushroom tyrosinase and cellular tyrosinase activities of oxyresveratrol: comparison with mulberroside A

The results indicate that the inhibition rate of mushroom tyrosinase might not provide an accurate estimate of the inhibition rates of melanin synthesis in melanocytes.

In vitro pharmacokinetic characterization of mulberroside A, the main polyhydroxylated stilbene in mulberry (Morus alba L.), and its bacterial metabolite oxyresveratrol in traditional oral use.

A key role of intestinal bacterial conversion in absorption and systemic exposure of MulA and its resultant bacterial metabolite OXY in oral route in humans and rats is revealed and warranted further investigational emphasis on OXY and its hepatic metabolites for understanding the benefits of mulberry.

Phytostilbenoid production in white mulberry (Morus alba L.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis

Results indicated that the glycoside mulberroside A in the cell is sensitive to the endogenous enzymatic hydrolysis, which has provided a new approach to producing glycosidic compounds and corresponding aglycones in cell culture.

Improvement of stilbenoid production by 2-hydroxypropyl-β-cyclodextrin in white mulberry (Morus alba L.) callus cultures

The results show that the addition of 2-hydroxypropyl-β-cyclodextrin significantly changed the patterns and levels of the stilbenoids in M. alba callus cultures.

Isolation, Identification, and Quantification of Tyrosinase and α-Glucosidase Inhibitors from UVC-Irradiated Mulberry (Morus alba L.) Leaves

Levels of these eight compounds were increased significantly following irradiation compared with untreated mulberry leaves; ORTs increased approximately 4 fold and moracins increased 2~16 fold, suggesting that UVC-IML may represent a promising source of nutraceuticals and cosmeceuticals for prevention of diabetes and skin aging.

The Study of Antioxidant and Antityrosinase Activity of Extract from Mulberry Root (Morus alba L.)

Objective: The objective of this study was to investigate the antioxidant and antityrosinase activities of the extract of mulberry root related to oxyresveratrol content and to find out that which



Enhancement of tyrosinase inhibition of the extract of Veratrum patulum using cellulase

It is indicated that deglycosylation of stilbene compounds has exerted more effective inhibition on the enzyme than that of the unprocessed plant extract.

Oxyresveratrol as the potent inhibitor on dopa oxidase activity of mushroom tyrosinase.

  • N. ShinS. Ryu Y. Kim
  • Biology, Chemistry
    Biochemical and biophysical research communications
  • 1998
The results indicate that both the number and positions of hydroxy groups in oxyresveratrol seem to play a critical role in exerting the inhibitory effect on dopa oxidase activity of mushroom tyrosinase.

Flavonols from saffron flower: tyrosinase inhibitory activity and inhibition mechanism.

A common flavonol, kaempferol, isolated from the fresh flower petals of Crocus sativus L. (Iridaceae) was found to inhibit the oxidation of L-3,4-dihydroxyphenylalanine (L-DOPA) catalyzed by mushroom

Oxyresveratrol and Hydroxystilbene Compounds

The depigmenting effect of oxyresveratrol works through reversible inhibition of tyrosinase activity rather than suppression of the expression and synthesis of the enzyme.

Microbial transformation and bioactivation of isoflavones from Pueraria flowers by human intestinal bacterial strains

The microbial transformation of the main isoflavones (1, 1a and 2) of Puerariae Flos is elucidated by using 29 commercially available human intestinal bacterial strains together with the bioactivation of the hepatoprotective activity of their metabolites.

Inhibition of tyrosinase by flavonoids, stilbenes and related 4-substituted resorcinols: structure-activity investigations.

Kinetic studies have indicated that specific compounds having the 4-substituted resorcinol skeleton exhibit competitive inhibition of the oxidation of DL-beta-(3,4-dihydroxyphenyl)alanine (DL-DOPA) by mushroom tyrosinase, which could lead to the design and discovery of new tyosinase inhibitors.

Biotransformation of common bean (Phaseolus vulgaris L.) flavonoid glycosides by bifidobacterium species from human intestinal origin.

Five strains of bifidobacteria, being active in the biotranformation of flavonoid glycosides occurring in common bean seeds and seedlings, could be considered as probiotic dietary adjuncts to improve the nutritional and health properties of Flavonoid-based products.

Oxyresveratrol as an antibrowning agent for cloudy apple juices and fresh-cut apples.

It was found, similar to 4-hexylresorcinol, that oxyresveratrol could effectively inhibit browning in cloudy apple juices at a concentration as low as 0.01% and that mulberry twig extract showed remarkable antibrowning effects on cloudy Apple juices.

Monoamine oxidase-A inhibitors from medicinal plants

Thirty kinds of medicinal plants were screened to examine inhibitory activities on rat brain monoamine oxidase A, using serotonin as a substrate and inhibitory potencies towards MAO-A of some stilbenes and flavonoids were also compared.