Biotination of proteins in vivo. A post-translational modification to label, purify, and study proteins.

@article{Cronan1990BiotinationOP,
  title={Biotination of proteins in vivo. A post-translational modification to label, purify, and study proteins.},
  author={John E. Cronan},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 18},
  pages={10327-33}
}
Post-translational modification of proteins with biotin provides the means to specifically label proteins in vivo and to purify proteins from crude cell lysates. The carboxyl-terminal protein segments modified by reaction with biotin ligase are strongly conserved in nature. We have demonstrated that the proteins encoded by translational gene fusions of a number of heterologous proteins to these carboxyl-terminal sequences become biotinated in vivo. The minimum size of the protein segment needed… CONTINUE READING