Biotin treatment causing erroneous immunoassay results: A word of caution for clinicians.

  title={Biotin treatment causing erroneous immunoassay results: A word of caution for clinicians.},
  author={Raashda A Sulaiman},
  journal={Drug discoveries \& therapeutics},
  volume={10 6},
  • R. Sulaiman
  • Published 2016
  • Medicine
  • Drug discoveries & therapeutics
Biotin or vitamin B7 when ingested in high doses may cause immunoassay interference leading to false potentially misleading results. It is important that clinicians should always interpret laboratory results in the context of patient's clinical state as erroneous results may lead to misdiagnosis and injudicious treatment with adverse patient outcome. 

Biotin interference in clinical laboratory tests

Effect of Biotin on Clinical Laboratory Test Results

  • A. Dasgupta
  • Biology
    Biotin and Other Interferences in Immunoassays
  • 2019

The effect of biotin interference on the results of blood hormone assays.

Practical recommendations and a procedure to be followed in the case of suspected interference of biotin in immunochemical assays, for clinicians and laboratory diagnosticians are presented.

AACC Guidance Document on Biotin Interference in Laboratory Tests.

The recent increase in the use of high-dose biotin supplements requires laboratorians and clinicians to be mindful of the potential for biotin interference in biotinylated immunoassay-based laboratory tests.

Population pharmacokinetics of exogenous biotin and the relationship between biotin serum levels and in vitro immunoassay interference

The effective half-life of biotin and biotin metabolites is evaluated, and a pharmacokinetic (PK) model is established to simulate the time taken for the biotin concentration to fall below a series of thresholds to avoid false assay results.

OP-JALM190029 575..587

  • 2020



Interference by Biotin Therapy on Measurement of TSH and FT4 by Enzymeimmunoassay on Boehringer Mannheim ES700 Analyser

For the routine screening of cord blood for congenital hypothyroidism, thyroid stimulating hormone (TSH)I is measures by enzyme immunoassay using the Boehringer Mannheim ES700 analyser using the cut off level of 30 mIU/L.

Depression in adult patients with biotin responsive basal ganglia disease.

Two patients with BBGD who were metabolically stable on treatment and developed depression later in life are reported to highlight the association of depression with basal ganglia disorders and demonstrate that depression is the potential long term complication of BBGD.