Biotechnology of flavonoids and other phenylpropanoid‐derived natural products. Part II: Reconstruction of multienzyme pathways in plants and microbes

  title={Biotechnology of flavonoids and other phenylpropanoid‐derived natural products. Part II: Reconstruction of multienzyme pathways in plants and microbes},
  author={Filippos Ververidis and Emmanouil A. Trantas and Carl J. Douglas and Guenter Vollmer and Georg Kretzschmar and Nickolas J. Panopoulos},
  journal={Biotechnology Journal},
Plant natural products derived from phenylalanine and the phenylpropanoid pathways are impressive in their chemical diversity and are the result of plant evolution, which has selected for the acquisition of large repertoires of pigments, structural and defensive compounds, all derived from a phenylpropanoid backbone via the plant‐specific phenylpropanoid pathway. These compounds are important in plant growth, development and responses to environmental stresses and thus can have large impacts on… 
Biosynthesis and Regulation of Phenylpropanoids in Plants
ABSTRACT Phenylpropanoids are a large class of plant secondary metabolites derived from aromatic amino acids phenylalanine in most plants or tyrosine in partial monocots. It mainly includes
Biosynthetic Pathway and Metabolic Engineering of Plant Dihydrochalcones.
The functions of genes and enzymes involved in the biosynthetic pathway of the dihydrochalcones are discussed and the recent strategies and achievements used in the reconstruction of multi-enzyme pathways in microorganisms in efforts to be able to attain higher amounts of desired diHydrochalcone compounds are discussed.
Flavonoid Biosynthetic Pathway: Genetics and Biochemistry
The deep knowledge of flavonoid regulation by micro-RNAs has been provided that attracts the biotechnologists to develop new molecular approaches so as to engineer various plant metabolic pathways to enhance the health-promoting metabolites in plants for human consumption.
Combinatorial Biosynthesis of Non-bacterial and Unnatural Flavonoids, Stilbenoids and Curcuminoids by Microorganisms
The concept of building and expressing artificial biosynthetic pathways for production of non-bacterial and unnatural compounds in microorganisms should be successfully applied to production of not only plant-specific polyketides but also many other useful compound classes.
Decreasing unpalatable flavonoid components in Citrus: the effect of transformation construct.
In an effort to alter the types and levels of flavanone neohesperidosides in citrus, an Agrobacterium-mediated genetic transformation approach was employed and transgenic plants with decreased leaf naringin levels were obtained.
Role of Flavonoid and Isoflavonoid Molecules in Symbiotic Functioning and Host-Plant Defence in the Leguminosae
The effects of root-nodule bacteria (hereafter called “rhizobia”) on the synthesis and release of flavonoid and isoflavonoid signal compounds are discussed, and the biological significance of phytoalexin production in legume plant nodulation and defense against pathogens and insect pests is explored.
Metabolomics Integrated with Transcriptomics Reveals Redirection of the Phenylpropanoids Metabolic Flux in Ginkgo biloba.
Comprehensive analysis of transcriptome and metabolite profiles of Ginkgo high-flavonoids mutant (ZY1) and Anlu1 (control) leaves revealed that the abundances of some major flavonoids were most significantly increased, while other phenylpropanoid-derived products and lipids showed the most largely reduced abundances.


Biotechnology of flavonoids and other phenylpropanoid‐derived natural products. Part I: Chemical diversity, impacts on plant biology and human health
The diversity and biosynthetic origins of phenylpropanoids and particularly of the flavonoid and stilbenoid natural products are discussed, referring on their effects on human health and physiology and their roles as plant defense and antimicrobial compounds.
Flavonoids and isoflavonoids - a gold mine for metabolic engineering.
Biosynthesis of plant-specific stilbene polyketides in metabolically engineered Escherichia coli
Phenylpropionic acids, such as 4-coumaric acid and caffeic acid, can be efficiently converted to stilbene compounds by recombinant E. coli cells expressing plant biosynthetic genes and optimization of precursor conversion and cyclization of the bulky ferulic acid precursor by host metabolic engineering and protein engineering may afford the synthesis of even more structurally diverse stilBene compounds.
Engineering isoflavone metabolism with an artificial bifunctional enzyme
Through a combined molecular modeling, in vitro protein engineering and in planta metabolic engineering approach, it was possible to increase the potential for accumulation of isoflavonoid compounds in non-legume plants.
Metabolic Engineering of Isoflavone Biosynthesis
Pathway engineering for healthy phytochemicals leading to the production of novel flavonoids in tomato fruit.
The results on genetic engineering of flavonoids in tomato fruit demonstrate the possibilities to change the levels and composition of health-related polyphenols in a crop plant and provide more insight in the genetic and biochemical regulation of the flavonoid pathway within this worldwide important vegetable.
Pleiotropic effect of phenolic compounds content increases in transgenic flax plant.
A significant increase in monounsaturated fatty acids and a slight increase in lignans content accompanied the increase in antioxidant properties of flaxseeds, indicating a strong relationship between phenolic acids content and antioxidant capacity.
Production of the isoflavones genistein and daidzein in non-legume dicot and monocot tissues.
Income is provided into requirements for metabolic engineering for isoflavone production in non-legume dicot and monocot tissues and the genistein produced in tobacco, Arabidopsis, and maize cells was present in conjugated forms, indicating that endogenous enzymes were capable of recognizing Genistein as a substrate.