Biosynthetic studies on the alpha-glucosidase inhibitor acarbose in Actinoplanes sp.: source of the maltose unit.

@article{Lee1997BiosyntheticSO,
  title={Biosynthetic studies on the alpha-glucosidase inhibitor acarbose in Actinoplanes sp.: source of the maltose unit.},
  author={S B Lee and B Sauerbrei and Jijrg Niggemann and Erin E. Egelkrout},
  journal={The Journal of antibiotics},
  year={1997},
  volume={50 11},
  pages={954-60}
}
To investigate the source of the maltose unit in acarbose, feeding experiments using 3H- or 2H-labeled maltose or maltotriose were carried out with resting cells of Actinoplanes sp. SN223/29. It was found by experiments with [6"-3H]- and [1-3H]maltotriose that a maltose unit from the nonreducing end of maltotriose is incorporated into acarbose more efficiently than from the reducing end. However, experiments with [6"-2H]- and [2-2H]maltotriose showed that maltose from either the reducing end or… CONTINUE READING