Biosynthetic response of cartilage explants to dynamic compression

@article{Sah1989BiosyntheticRO,
  title={Biosynthetic response of cartilage explants to dynamic compression},
  author={Robert L. Sah and Young-Jo Kim and J. Y. H. Doong and Alan J. Grodzinsky and Anna Plass and John D. Sandy},
  journal={Journal of Orthopaedic Research},
  year={1989},
  volume={7}
}
The biosynthetic response of calf articular cartilage explants to dynamic compression was examined over a wide range of amplitudes, waveforms, and frequencies. Glycosaminoglycan synthesis was assessed by 35S‐sulfate incorporation, and amino acid uptake and protein synthesis were assessed by 3H‐proline incorporation. Two culture chambers were designed to allow uniaxial radially unconfined compression and mechanical testing of cartilage disks: one chamber was used inside a standard incubator; the… 
Mechanical compression modulates matrix biosynthesis in chondrocyte/agarose culture.
TLDR
The fact that chondrocyte response to static compression was significantly affected by the presence or absence of matrix, as were the physical properties of the disks, suggested that cell-matrix interactions may be more important than matrix-independent cell deformation and transport limitations in determining the biosynthetic response tostatic compression.
Growth Responses of Cartilage to Static and Dynamic Compression
TLDR
Results indicate that synthesis of glycosaminoglycan and deoxyribonucleic acid, two distinct indices of cartilage growth, are regulated independently by mechanical loading and that cartilage responds differently to static and dynamic loading at different stages of maturation.
Collagen synthesis of articular cartilage explants in response to frequency of cyclic mechanical loading
Articular cartilage in vivo experiences the effects of both cell-regulatory proteins and mechanical forces. This study has addressed the hypothesis that the frequency of intermittently or
Biosynthetic response of passaged chondrocytes in a type II collagen scaffold to mechanical compression.
TLDR
The general biosynthetic response of passaged chondrocytes in the porous type II collagen scaffolds is similar to that seen for chondrosine in their native environment, and future work needs to be directed to modifications of the cell-seeded construct to allow for the capture of the newly synthesized matrix molecules by the scaffold.
Characterization of cartilage metabolic response to static and dynamic stress using a mechanical explant test system.
Biosynthetic response and mechanical properties of articular cartilage after injurious compression
Static and dynamic compression modulate matrix metabolism in tissue engineered cartilage
Effect of intermittent cyclic preloads on the response of articular cartilage explants to an excessive level of unconfined compression
TLDR
Low intensity, intermittent cyclic loading was applied to chondral explants prior to an acute unconfined compression on the tissue, showing cyclic preloading increased the proteoglycan content and mechanically stiffened the explants, making them more resistant to matrix damage and cell death under 25 MPa of unconfining compression up to 14 days.
Compressive strains at physiological frequencies influence the metabolism of chondrocytes seeded in agarose
  • D. Lee, D. Bader
  • Biology
    Journal of orthopaedic research : official publication of the Orthopaedic Research Society
  • 1997
TLDR
The three parameters investigated were each influenced by the dynamic strain regimens in a distinct manner, implying that the signalling mechanisms involved are uncoupled.
Static and dynamic compression regulate cartilage metabolism of PRoteoGlycan 4 (PRG4).
TLDR
Immunohistochemistry revealed that all compression protocols also affected the number of cells expressing PRG4, suggesting the paradigm that mechanical stimuli regulate biosynthesis in cartilage appears operative not only for load bearing matrix constituents, but also forPRG4 molecules mediating lubrication.
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References

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TLDR
It is suggested that compression‐induced changes in local, interstitial pH may account for the observed chondrocyte biosynthetic response to static compression.
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TLDR
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TLDR
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TLDR
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TLDR
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TLDR
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