Biosynthetic method for introducing unnatural amino acids site-specifically into proteins.

@article{Ellman1991BiosyntheticMF,
  title={Biosynthetic method for introducing unnatural amino acids site-specifically into proteins.},
  author={J. Ellman and D. Mendel and S. Anthony-Cahill and C. Noren and P. Schultz},
  journal={Methods in enzymology},
  year={1991},
  volume={202},
  pages={
          301-36
        }
}
Publisher Summary This chapter discusses a biosynthetic method for introducing unnatural amino acids site-specifically into proteins. This method involves the replacement of the codon for the amino acid of interest with the amber nonsense codon by conventional oligonucleotide-directed mutagenesis. The amber nonsense codon is not recognized by any of the common tRNAs involved in protein synthesis, and thus can be viewed as a “blank” in the genetic code. A suppressor tRNA is then constructed that… Expand
Expanding the genetic code.
Site‐Specific Incorporation of Unnatural Amino Acids into Proteins
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Incorporation of nonnatural amino acids into proteins.
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